Evaluation of erythrocyte viability, antioxidant capacity and antiplasmodial activity induced by alkaloid extract of Phyllanthus amarus

Introduction: The production of large amounts reactive oxygen species in severe malarial infection is due to parasite invasion erythrocytes. Malaria resistance medication has left malaria-endemic countries with no alternate source medications but traditional medicine. One such plants utilized by healers Phyllanthus amarus. Therefore, this study aims at ascertaining the antiplasmodial and cytotoxic activities P. amarus its specific actions on retaining erythrocyte viability antioxidant activity. Methods: Antiplasmodial were determined vitro via suppression tetrazolium-based colorimetric assays, respectively. Antioxidant capacity was measuring extract’s ability inhibit lipid peroxidation, scavenge 1,1-diphenyl-2-picrylhydrazyl (DPPH) superoxide ions, reduce iron(III) chelate iron using documented methods. Results: Alkaloid extracts showed great activity (IC50=0.52μg/mL) low (CC50=54.95 μg/mL). Erythrocyte assay minimal impact extract uninfected erythrocytes improved infected RBC a dose dependent manner, manifested mainly chelating (EC50=0.34 Conclusion: This suggests that alkaloid significant activities. These promoted repair parasite-induced free radical damage membrane distorted parasites redox balance defense mechanism, hence survival rate as indicated associated treatment infection.